Biostatistics Seminar: Transcription factor target identification via knock-down experiments

Please join the Biostatistics Seminar October 22, 2018 at 12:00 Noon at 60 College Street, Rm. 216. Please also refer to the flyer attached.

Thank you.

Speaker: Leying Guan

Institution: Stanford University

Time & Place: 12:00 Noon in Room 216, 60 College Street

Title: “Transcription factor target identification via knock-down experiments”


The perturbation of a transcription factor should affect the expression levels of its direct targets. However, not all genes showing changes in expression are direct targets. To increase the chance of detecting direct targets, we propose two new methods. The first one is a modified two-group model using data from a single knock-down experiment. The null group corresponds to genes that are not direct targets, but can have small non-zero effects. The second method constructs a depth constrained network using data from multiple knock-down experiments. I will give details of both methods and present some simulated as well as real data examples.

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